———–快要快要结束结束了了,还有还有难熬难熬的的几天几天;
       想要想要结束了结束了,想要想要不结束不结束.

Aug 18 Mon sunny w/ evening storm

9:00-10:15: Restreak ESTM 32, 39~41 from glycerol stock.
10:15-12:50: ESTM retest: Inoculate plants with .0002 bac. strains.
13:40-14:30: Recover plasmid DNA from bac. solution using alkaline buffer with SDS.
14:30-15:15: Prepare MS media for Col-0 seedling plates.
15:15-15:25: Data recording.
15:25-16:15: ESTM growth assay Day 0 set up: Obtain leaf tissue solution from three samples of each strain and plate out the dilution series.
16:15-16:40: PCR set up for the extracted plasmid DNA.
16:40-17:10: PCR set up for initial bac. strains digested with another set of restriction enzyme (E/P).

———–在在疲惫不堪疲惫不堪中中这一周这一周终于终于结束结束了了.因为因为一些一些念头念头总是总是萦绕萦绕在在脑海脑海的的缘故缘故,做起事做起事来来总是总是心不在焉心不在焉,干什么干什么都都费力极了费力极了.希望希望赶紧赶紧暂停暂停一下一下喘口气喘口气.不知道不知道这种这种状态状态还会还会持续持续多久多久.或许或许很久很久.
———–周五周五下午下午实验室实验室照例照例没什么没什么人人.当我当我整理完整理完笔记笔记跟跟karl道别道别的的时候时候,窗外窗外的的天气天气和和实验室实验室冷冰冷冰的的气氛气氛和和他他空漠空漠的的眼神眼神,让我让我有些有些说不出话说不出话来来.像是像是离别离别的的时刻时刻,又又或许或许是是失败失败的的预兆预兆.我我有些有些害怕害怕.
———–手手破了破了以后以后,就得用就得用一只手一只手干活干活了了.单手单手洗脸洗脸.单手单手刷牙刷牙.单手单手切菜切菜.单手单手洗澡洗澡.单手单手打枪打枪.单手单手盖被子盖被子.看来看来这样这样的的生活生活也也并非并非不能不能过过.
———–耳机耳机终于到了终于到了.终于终于有有个个好消息好消息.拍了拍了几张几张照片照片.
———–傍晚傍晚的的时候时候究竟究竟还是还是下雨下雨了了.只能只能看书看书.我我现在现在只想只想做一个做一个有理想有理想有道德有道德的的人人.这这终究终究太难了太难了.
———–DALA.我是我是光明正大光明正大的的.

Aug 15 Fri sunny w/ evening storm

9:00-10:50: Prepare Col-0 seedling plates.
10:50-11:40: Colony PCR.
11:40-12:50: ESTM 31~34 check and set up retest.
13:40-16:00: ESTM retest using .004 bac. strains.
16:00-16:30: Run gel using colony PCR product.
16:30-17:20: Wash plates.
17:20-17:40: Gel result checking.

Aug 14 Thur overcast

9:00-13:00: ESTM 35&36 inoculation expt.
14:00-17:00: ESTM 37&38 inoculation expt.
17:00-17:10: Restreak bac. strains for retesting.

———–OBSESSION MAKES ME ANXIOUS AND EXHAUSTED.
       IM NOWHERE TO HIDE WHEN INFATUATION COMES AROUND.
———–TE DESEO, TARA.

Aug 13 Wed rainy

9:00-9:55: Prepare gel.
9:55-10:20: Restreak ESTM 35~38.
10:25-11:00: Run gel.
11:00-11:20: Rack tips.
11:20-12:00: Gel result checking.
12:45-13:25: Gel result checking.
13:30-13:55: eXtract DNA from gel and prepare for cloning.
13:55-14:15: Wash plates.
14:25-14:50: Cloning setup.
14:50-15:45: Wash trays.
16:00-17:00: Rack tips.

Aug 12 Tue sunny

9:00-10:15: Restreak ESTM 35~38 from glycerol stocks.
10:15-11:00: PCR set up.
11:00-13:10: Collect leaf tissue from ecotype mutants and freeze in liquid nitrogen.
13:10-13:25: Prepare gel.
14:00-14:30: Run gel for forty mins.
14:30-15:20: Finish up collecting leaf tissue.
15:20-16:00: Gel result checking.
16:00-16:50: Gradient PCR set up.