Aug 12 Tue sunny

9:00-10:15: Restreak ESTM 35~38 from glycerol stocks.
10:15-11:00: PCR set up.
11:00-13:10: Collect leaf tissue from ecotype mutants and freeze in liquid nitrogen.
13:10-13:25: Prepare gel.
14:00-14:30: Run gel for forty mins.
14:30-15:20: Finish up collecting leaf tissue.
15:20-16:00: Gel result checking.
16:00-16:50: Gradient PCR set up.