Day 53

August 2, 2008 — 日记独白 — 1 min read

Aug 1 Fri sunny

9:00-11:00: Prepare Col-0 seedling plates.
11:00-11:20: Count colonies from ESTM growth assay Day 0.
11:30-12:20: Wash trays.
12:05-12:50: Prepare KB media and go autoclaving.
13:30-14:00: Autoclave out.
14:00-15:00: Wash plates.
15:00-15:50: Prepare KB media plates with antibiotics.
16:00-16:15: ESTM growth assay Day 3: Make dilution series and plate out.

Day 52

August 1, 2008 — 日记独白 — 1 min read

July 31 Thur sunny

9:00-10:50: ESTM retesting: Make dilution series.
11:00-13:25: Inoculate plates with .004 baceteria strains.
13:30-14:45: Wash plates.
14:45-15:30: Rack tips.
16:00-16:25: ESTM retest: Make dilution series for 8B7/10B7/524.

Day 51

July 30, 2008 — 日记独白 — 1 min read

July 30 Wed sunny

9:00-13:10: ESTM 27&28 inoculation expt.
13:40-16:35: ESTM 29&30 inoculation expt.
16:40-18:00: Make dilution series for bacteria solution interested and plate out on petri dish.
18:00-18:15: Restreak bacteria strains for retesting.

Day 50

July 30, 2008 — 日记独白 — 1 min read

July 29 Tue sunny

9:00-9:50: Wash plates.
9:50-10:30: Restreak bacteria strains for retesting.
10:30-11:00: ESTM Growth Assay in Seedling Media recording (22 hpi).
11:00-11:30: Rack tips.
11:30-12:00: Restreak 8B7/10B7/524 for retesting.
13:00-13:25: Restreak ESTM 27~30.
13:30-15:55: Harvest seeds.
16:00-17:00: Prepare MS media for Col-0 seedling plates.

Day 49

July 29, 2008 — 日记独白 — 1 min read

July 28 Mon sunny

9:00-12:10: ESTM Growth Assay in KB/seedling media preparation for 8B7/10B7/524 (ctrl).

11:00-11:20: Col-0 seedling plates sterilization.

13:10-13:50: KB media preparation.

13:55-14:30: Restreak ESTM 27~30 from glycerol stocks.

14:30-15:30: Prepare media plates for Col-0 seedling (14).

15:40-17:50: Prepare KB media plates with antibiotics.

17:50-18:00: ESTM Growth Assay in KB media recording.