Aug 11 Mon sunny

9:00-10:30: Prepare KB&LB media and go autoclaving.
10:30-11:05: PCR set up.Sterilize Col-0 seedling plates.
11:05-11:35: Wash plates.
11:35-12:00: Autoclave out.
12:00-12:35: Restreak bacteria strains for retest.
13:25:-13:55: PCR done. Run gel for 40 mins.
13:55-14:55: Prepare MS media stocks for Col-0 seedling plates.
14:55-15:30: Electrophoresis results checking.

Aug 8 Fri sunny

9:00-11:30: Prepare Col-0 seedling plates.
11:30-12:00: Wash plates.
12:00-12:25: Prepare gel for electrophoresis.
13:00-13:35: ESTM retest recording.
13:35-14:35: Wash plates./Electrophoresis result checking.
14:40-15:10: PCR using universal vector.
15:10-18:00: Wash trays.
18:00-18:25: ESTM retest (Day 3): Make dilution series and plate out.

Aug 7 Thur sunny

9:00-10:00: Rack tips.
10:00-13:10: ESTM 31&32 inoculation expt.
14:00-16:30: ESTM 33&34 inoculation expt.
16:30-18:30: ESTM retest: inoculate Col-0 plates with .004 bacteria strains.
18:30-19:05: ESTM retest (Day 1): Make dilution series and plate out.

Aug 6 Wed sunny

9:00-10:00: Restreak ESTM 31~34.
10:00-11:10: PCR set up for 8B7 (with universal vector).
11:10-11:30: Wash plates.
11:30-12:00: Paper review.
13:00-13:30: Paper review. Wash plates.
13:30-14:00: Run electrephoresis for PCR products.
14:00-14:30: ESTM 27~30 checking.
14:30-15:35: ESTM retest: make dilution series and plate out.
15:35-16:15: Second PCR set up with vetor nested.
16:15-18:20: Collect seedling media from Col-0 plates and prepare ESTM growth assay.
                  Restreak bacteria strains.

Aug 5 Tue sunny w/ eveing storm

9:00-10:00: Wash plates.
10:00-10:30: Restreak ESTM 31~34.
10:30-10:40: Col-0 seedling plates sterilization.
10:40-11:05: ESTM growth assay recording.
11:20-12:00: Restreak bacteria strains for retesting.
13:00-14:50: Transplant Col-0 and Ecotype #8/10/74.
14:50-15:30: Prepare MS media stocks for Col-0 seedling plates.
15:30-16:10: shaker checking.