Thur May 15 sunny

9:00-10:00: Transfer seeds.
10:00-11:00: Lab meeting. Two paper discussed in DD & DG lab.
11:00-12:00: Transfer seeds cont’d.
12:40-13:40: Harvest seeds.
13:50-14:50: Transfer seeds.
15:00-17:00: Replica plate the colonies from selective plates incubated to new plates with the same two antibiotics.
17:00-18:00: Transer seeds.

Wed May 14 overcast afternoon shower

9:00-9:40: Wash 96-well plates.
9:50-11:00: Make bacteria freeze stocks. (glycerol added as anti-crystallization chemical; freeze with liquid nitrogen)
11:00-13:00: Harvest seeds.
14:00-15:10: Harvest seeds cont’d.
15:20-17:10: Transfer seed samples.

Day 1 May 12 Mon overcast
9:00-10:30 Make E. coli replica plate (2).
10:30-13:00 Harvest seeds.
14:00-15:00 Harvest seeds (Cont’d).
15:00-15:30 Rinse and dry trays.
15:30-16:00 Transposon experiment: Part 1 Conjugation: Mix strains onto plain KB plates. Incubate overnight (30 C).
16:00-17:10 Harvest seeds (one more tray).

Day 2 May 13 Tue sunny
9:00-10:10 Wash 96-well plates.
10:10-11:00 Transposon experiment: Part 1 Conjugation: replica-plate strains from plain KB plate onto selective plate. Incubate at 30 C.
11:00-12:00 Harvest seeds (one more).
13:00-15:00 Wash and dry trays (several tons).
15:00-17:00 Make bacteria stock in 96-well plates (3) from replica plates did yesterday. Shake overnight.