Day 1 May 12 Mon overcast
9:00-10:30 Make E. coli replica plate (2).
10:30-13:00 Harvest seeds.
14:00-15:00 Harvest seeds (Cont’d).
15:00-15:30 Rinse and dry trays.
15:30-16:00 Transposon experiment: Part 1 Conjugation: Mix strains onto plain KB plates. Incubate overnight (30 C).
16:00-17:10 Harvest seeds (one more tray).
9:00-10:30 Make E. coli replica plate (2).
10:30-13:00 Harvest seeds.
14:00-15:00 Harvest seeds (Cont’d).
15:00-15:30 Rinse and dry trays.
15:30-16:00 Transposon experiment: Part 1 Conjugation: Mix strains onto plain KB plates. Incubate overnight (30 C).
16:00-17:10 Harvest seeds (one more tray).
Day 2 May 13 Tue sunny
9:00-10:10 Wash 96-well plates.
10:10-11:00 Transposon experiment: Part 1 Conjugation: replica-plate strains from plain KB plate onto selective plate. Incubate at 30 C.
11:00-12:00 Harvest seeds (one more).
13:00-15:00 Wash and dry trays (several tons).
15:00-17:00 Make bacteria stock in 96-well plates (3) from replica plates did yesterday. Shake overnight.