Day 13 (外一篇)

— Posted in日记独白 — 1 min read
Jun 4 Wed overcast
9:00-14:00: Inoculate Col-0 with ESTM 3&4 (three replica each).
15:00-16:00: Harvest seeds.
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蚂蚁的品性
蚂蚁是天生的勤劳者.它们是自然界中的大力士,可以负起超过身体数倍的物体.孩子们喜欢看蚁群爬上一块石头又掉下来.这些天然的品性足以值得称道.于是蚂蚁成了质朴的劳动人民的代表.它们不求索取,百折不挠,又繁衍不息.真真像是我们的民族呵.
 
不过愈是伟大的性格也愈容易变得卑劣.吃苦耐劳在虚荣的驱使下,会变得形式与不切实际;坚忍不拔在失败的面前,亦会变得暴戾无常.
 
因此,愈是平凡而显得伟大的种族,或许愈容易遇到危险.只消一小块糖,就可以黏住一群蚂蚁;一丝光亮,亦可以吸引许多飞蛾.不过幸好它们的繁殖能力颇强,这些损失亦非不能弥补.
 
当然,蚂蚁或许是会便聪明的.但是变聪明不等于有思想.只要些微高明点的伪装,亦能灭亡它们.并且开了窍的那些,便会愈感到自己的伟大,因此每每在遇到灾难时候,便总觉得上天的不公,于是变得愈加冲动,易被利用了.
 
蚁群里倘若真的有一股力量,那么有头脑,能洞悉这一切,它们或许会暗自高兴不已.因为这实在是一支再好不过的武器了.只要稍许挥动一下旗帜,庞大的蚁群便成了无坚不摧的激光,即使是石头,也能烧出个洞来.
 
其他蚂蚁自然是不知道的,它们会为自己身在这样的洪流中感到自豪,甚至有些骄傲,当取得了这样那样的胜利果实之后;于是便愈加容易被驱使了.世界上没有爬不上的石头,因为我们有坚忍不拔的品格;世界上没有什么是不可想象的,因为我们是那样庞大的群体;也没有任何别的势力能摧毁我们,因为我们的目光犀利,我们的战斗力顽强.
 
认识到团结的力量,它们更加注重内部的联合.于是面对或自然或外来群落的袭击时,一场热火朝天的自救行动展开了.它们深知,要从废墟里站起来,自信是最不可缺少的,即使是盲目的自大也被看作鼓舞的力量.同时,对别的动物的抨击之声也是一剂强效的安慰剂.它们把建筑推倒再搭起来,一如不停的爬上石头再跌下来;每天靠着对外面传来的声音的咒骂和对自我的夸赞度日;建设着自己的乌托邦中焦躁不安,深怕自己太渺小.
 
当然,象蚁群俯视着的老鼠决计不会知道它们的宏图大业,只消一舌头下去,再舔舔河水,饱睡一觉,就满足了.
 
 
 
 

Day 12

— Posted in未分类 — 1 min read
Jun 3 Tue Sunny w/ early shower

9:00-9:50: ML1 Insertion activation line 61~121 preparation: Tape the number tag.
9:50-10:50: Make ecotype stocks (3 and 4).
10:50-11:00: Set up plate sterilization (as described before).
11:00-11:40: Wash and rinse plates.
11:40-12:00: Wash and dry petri dishes.
12:00-13:40: Inoculate plants with 0.1A ES524 using syringe.
14:20-17:30: Make ecotype 3&4, ML1 Insertion 61~121 stocks in 96-well plates:
ecotype: Add 200 micro litre MS+MES media to two plates with ecotype seeds.
ML1: 1) Add 250 micro litre media to each tube with seeds;
          2) Transfer 50 micro litre solution from each tube to the well of 96-well plates (four replica prepared);
          3) Add 150 micro litre media to each well.
          4) Add 200 micro litre media to the outer wells without seeds.
Stick aroud the plates and freeze in fridge.

17:30-18:10: Transfer leaf tissue from inoculated plants to petri dish to obtain bacteria growth curve.

Day 10

— Posted in日记独白 — 1 min read
May 30 Fri overcast w/ noon shower

9:00-12:00: Wash and dry trays and tubes used for seed harvesting.
In the mean time, 10:00-10:30: Autocleaving training session held.
12:00-12:30: Karl’s 24th b-day party (strawberry cake + pineapple).
12:30-18:45: bacteria inoculating experiment:
1) Transfer bacteira colony from petri dish to two 96-well plates labelled ESTM 1&2.
2) Calibrate bacteria concentration in two 96-well plates to be 0.1(?) with specially-designed plates and Optima spectrum reader and by adding proper amount of bacteria and MS + MES media (very time-consuming).
3) Dilute concentrations of two plates to 0.004 by mixing 8 micro-litre bacteria solution with 192 micro-litre MS+MES media.
4) Inoculate bacteria to Col-0 seedling plate 1and 2 (prepared in week 1)by adding 10 micro-litre diluted bacteria solution to each of the well. Three replicates are made for each of the plate.
18:50-19:30: CSB spring BBQ (RW010). Coming too late, with only cake and pop avail.

END OF WEEK TWO (PROBABLY GO TO LAB ON SAT.).

Day 9

— Posted in日记独白 — 1 min read
9:00-10:00: Make replica plates for ESTM1 & 2 (fix a few spots).
10:-10:15: Lab meeting (the presenter didnt show up).
10:15-11:45: Make minimum media:
                       KB media (bacteria media): 200 ml ×2, 600 ml×1 (peptone +phosphate +glycerol +agar).
                       Plant media: MS +MES +(agar).
                       Autoclave the liquid solutions prepared.
11:50-12:10: Rinse 96-well plates.
12:35-13:15: Sort tips into tip boxes.
13:15-14:20: Finish making KB and plant media (add 1M MgSO4, antibiotics Kan &ST). Transfer all solutions to petri dishes.
14:20-16:20: Sort tips into tip boxes.