Day 10

May 30 Fri overcast w/ noon shower

9:00-12:00: Wash and dry trays and tubes used for seed harvesting.
In the mean time, 10:00-10:30: Autocleaving training session held.
12:00-12:30: Karl’s 24th b-day party (strawberry cake + pineapple).
12:30-18:45: bacteria inoculating experiment:
1) Transfer bacteira colony from petri dish to two 96-well plates labelled ESTM 1&2.
2) Calibrate bacteria concentration in two 96-well plates to be 0.1(?) with specially-designed plates and Optima spectrum reader and by adding proper amount of bacteria and MS + MES media (very time-consuming).
3) Dilute concentrations of two plates to 0.004 by mixing 8 micro-litre bacteria solution with 192 micro-litre MS+MES media.
4) Inoculate bacteria to Col-0 seedling plate 1and 2 (prepared in week 1)by adding 10 micro-litre diluted bacteria solution to each of the well. Three replicates are made for each of the plate.
18:50-19:30: CSB spring BBQ (RW010). Coming too late, with only cake and pop avail.

END OF WEEK TWO (PROBABLY GO TO LAB ON SAT.).

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