Day 17

— Posted in日记独白 — 1 min read
Jun 11 Wed overcast

9:00-17:00: Make ESTM 5&6 inoculation plates as described before.
17:05-17:50: Make 10, 100, 1,000 and 10,000 dilution series for following inoculated solutions from ESTM 3 plates and plate and aliquot of each dilution onto a petri dish to observe bacteria growth: G3 (no bleached phenotype which is the phenotype in interest); D6 (bleached phenotype as positive control)

Day 16

— Posted in日记独白 — 1 min read
Jun 9 Mon Sunny w/ afternoon t-storm

9:00-9:50: Wash 96-well plates.
9:50-10:30: Plate the bacteria from glycerol stocks ESTM 5&6 to new plates.
10:30-11:45: Transplant Col-0 from pot to tra with soil (2).
11:45-12:10: Wash and rinse 96-well plates.
13:00-13:40: Bacteria colony count for leaf tissue plate prepared on Friday.
13:40-15:00: Harvest seeds.
15:20-15:40: Recycle the beads in leaf tissue solutions in tubes using sieve.
15:40-16:25: Make seedling stocks (Col-0 with transposon inserted)in 96-well plates by adding 200 micro litre MS+MES media to each well and freeze for storage.

Day 15

— Posted in日记独白 — 1 min read
9:00-10:15: Treat seedling plates with constitutive promoter transposon inserted with DMSO or Smex (two plates for each kind).
                     Smex is light sensitive.
10:15-10:50: ESTM 1&2 inoculation line result recording.
10:50-11:55: Make 10, 100 and 1,000 dilution of selected ESTM line wells and to observe bacteria growth by taking an aliquot of each one (5 micro litres) onto a petri dish.
12:00-13:40: Make 10, 100, 1,000 and 10,000 dilutions of solution of leaf tissue syringed by bacteria Tuseday (#5-16). Take an aliquot (5 micro litres) of each dilution onto petri dish to record bacteria growth.
14:20-17:00: Make Col-0 stock in 96-well plates with 200 micro litre MS+MES media (28 plates).
17:00-17:45: Add diluted pathogen #524 solution to plates with ecotype 1&2.
                       Add diluted pathogen #500 solution to ML1 activation line in DMSO or Smex media prepared this morning.

Day 14

— Posted in日记独白 — 1 min read
Jun 5 Thur overcast w/ afternonn shower
 
9:00-11:20: Make Col-0 stocks in 96-well plates (21 prepared).
11:30-13:40: Make sterile stocks for Col-0, NahG and six other mutants in 96-well plates.
14:10-14:40: Transplant excess Col-0 seeds and ecotype #8,10 and 74 to soil pots and grow in growth room.
14:40-16:15: Sort tips.
 
附记:
1)今天Mike也度假去了,于是不再有每天不间断播放的歌曲了.下午天也变得阴沉,实验室于是便愈发的冷清了.
一边整理着塑料滴管一边听着西海岸幽冷的爵士乐,百无聊赖之余,边想着昨天看的<闪开,让我歌唱八十年代>里有关毛片和麻将的回忆.
即便普通的文字,在说着我们重复经历的故事的时候,却也可发一噱.
秋天或许总会到来,抚平我们的脉搏;
可是秋天之前,总会有密不透风的低压,让我们心跳加快,期待着大雨将至;也会有终于来的交加的雷雨,让我们沸腾.
我们试图否定着,一直喃喃着"不.不";我们试图改变着,呼喊着"去死吧";我们期待着,渴望着,走的是一条新的,哪怕布满荆棘的路…
我们无力对抗倒退的洪流,终于发现我们还将是我们试图否定的,改变的人和人生.
迷路的人,总会不停的回到原点,纵使筋疲力尽.
但是我仍然要否定别人的肯定.
就像迷路的人.
明天会是忙碌的一天.
也会是炎热的一天.
 
2)真理终将夺得冠军.