9:00-10:15: Treat seedling plates with constitutive promoter transposon inserted with DMSO or Smex (two plates for each kind).
Smex is light sensitive.
10:15-10:50: ESTM 1&2 inoculation line result recording.
10:50-11:55: Make 10, 100 and 1,000 dilution of selected ESTM line wells and to observe bacteria growth by taking an aliquot of each one (5 micro litres) onto a petri dish.
12:00-13:40: Make 10, 100, 1,000 and 10,000 dilutions of solution of leaf tissue syringed by bacteria Tuseday (#5-16). Take an aliquot (5 micro litres) of each dilution onto petri dish to record bacteria growth.
14:20-17:00: Make Col-0 stock in 96-well plates with 200 micro litre MS+MES media (28 plates).
17:00-17:45: Add diluted pathogen #524 solution to plates with ecotype 1&2.
Add diluted pathogen #500 solution to ML1 activation line in DMSO or Smex media prepared this morning.
Smex is light sensitive.
10:15-10:50: ESTM 1&2 inoculation line result recording.
10:50-11:55: Make 10, 100 and 1,000 dilution of selected ESTM line wells and to observe bacteria growth by taking an aliquot of each one (5 micro litres) onto a petri dish.
12:00-13:40: Make 10, 100, 1,000 and 10,000 dilutions of solution of leaf tissue syringed by bacteria Tuseday (#5-16). Take an aliquot (5 micro litres) of each dilution onto petri dish to record bacteria growth.
14:20-17:00: Make Col-0 stock in 96-well plates with 200 micro litre MS+MES media (28 plates).
17:00-17:45: Add diluted pathogen #524 solution to plates with ecotype 1&2.
Add diluted pathogen #500 solution to ML1 activation line in DMSO or Smex media prepared this morning.