Day 27

June 24, 2008 — 日记独白 — 1 min read

9:00-10:00: Replicate ESTM 9&10.
10:00-12:40: Inoculate Col-0 plates with eight strains from ESTM line prepared at .004 concentration.
13:00-13:55: Wash 96w plates.

Day 26

June 24, 2008 — 日记独白 — 1 min read

9:00-15:00: ESTM growth assay set up: make 0.15 plate for each one tested in KB media.
15:00-15:30: Restreak ESTM 9&10.
15:30-17:10: Make .004 plate of pathogen #524 in MS media to inoculate ECO #6 and #500 for DMSO and Smex treated plates.
17:10-17:30: ESTM growth assay record (two hrs pt record).

Day 25

June 21, 2008 — 日记独白 — 1 min read

Jun 20 Fri overcast

9:00-11:00: Make Col-0 stock in 96w plates (20).
11:00-12:00: Paper review.
12:40-14:40: Make bacteria growth media from Col-0 media stock.
14:40-15:10: Wash 96w plates.
15:10-16:10: Make .004 pathogen #524 media and inoculate it to Ecotype #5.
16:10-17:00: Restreak bacteria from ESTM plates with non-bleached phenotype to petri dish.

Day 24

June 20, 2008 — 日记独白 — 1 min read

Jun 19 Thur overcast

9:00-10:00: eXamine ML161~121 w/ DMSO or Smex plates and ecotype 3&4 for non-bleached phenotype.
10:00-11:00: Lab meeting resumed.
11:00-11:40: Meeting with Darrell.
11:40-12:25: Rack tips and record examining results in computer.
13:00-13:50: Rack tips.
13:50-14:35: Wash 96w plates.
14:35-15:20: Rack tips.

Day 23

June 19, 2008 — 日记独白 — 1 min read

Jun 18 Wed overcast

9:00-10:30: ESTM 5&6 inoculation plate recording for plants w/ non-bleached phenotype.

Make dilution series for these wells as well as the negative controls to observe bacteria growth.

10:30-12:00: ESTM 7&8 inoculation prep: make .004 bacteria plates.

12:40-15:30: ESTM 7&8 inoculation

15:30-16:20: Ecotype retest seeding prep: pour seeds onto petri dish w/ MSagar media and dry.

16:20-16:50: Wash 96w plates.