July 11 Fri sunny 9:00-11:30: Prepare Col-0 seedling stocks (28).11:30-12:15: Restreak bacteria 524/782/783.12:45-18:15: Restreak bacteria 524/782/783.

July 10 Thur sunny 13:00-15:00: Make dilution series for leaf tissue solution obtained from plants inoculated with 3G3/5F8/524 on Monday. And plate out aliqouts onto petri dish.15:00-16:45: ESTM retest: inoculation with 2E5/7G10/8B7/10B7 and 524 (control).16:45-17:50: Restreak bacteria 524/782/783.

July 9 Wed sunny 9:00-13:35: ESTM 15&16 inoculation exp.14:10-16:40: ESTM 17&18 inoculation exp.16:40-17:05: Wash plates.17:05-17:20: ESTM growth assay recording.17:20-17:50: Make dilution series of ESTM 14 C7 (non-bleached phenotype) and F8 (control). And plate out aliquots onto petri dish to observe bacteria growth.

July 8 Tue sunny w/ evening t-storm 9:00-10:30: ESTM 15-18 restreak; Tri-parental mating: Spread bacteria growing on the media prepared yesterday onto eight plates.10:30-12:30: Transplant ecotype retest seedlings from petri dishes to soil pots.13:10-15:10: Wash and dry trays.15:10-16:05: Harvest seeds.

July 7 Mon sunny 9:00-10:25: ESTM inoculation preparation: #15-18.10:25-10:50: Col-0 plate sterilization set up.10:50-11:20: Tri-parental mating set up.11:20-11:35: Record ESTM 9&10 results.11:35-12:45: .0002 524/3G3/5F8 inoculum preparation.13:30-14:40: Inoculate plants in soil w/ inoculum using syringe.14:40-15:25: Prepare MS+MES media stocks for Col-0.15:25-16:00: Wash plates.16:00-16:40: Make inoculation […]