Day 34

July 7 Mon sunny

9:00-10:25: ESTM inoculation preparation: #15-18.
10:25-10:50: Col-0 plate sterilization set up.
10:50-11:20: Tri-parental mating set up.
11:20-11:35: Record ESTM 9&10 results.
11:35-12:45: .0002 524/3G3/5F8 inoculum preparation.
13:30-14:40: Inoculate plants in soil w/ inoculum using syringe.
14:40-15:25: Prepare MS+MES media stocks for Col-0.
15:25-16:00: Wash plates.
16:00-16:40: Make inoculation experiment Day-0 growth assay. And grow rest of the plants in growth chamber.

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