Day 67

—–What a numb day.
—–斜斜的阳光照在我身上.我的眼睛几乎睁不开来.我很害怕.或许我已经盲了.或许眼睛只是一个摆设.我已经看不见很多东西.或许…let the blind lead those who can see but cannot feel.
—–I wonder whether you can see/Inside my shell I wait and bleed.
                                                                             ———————-I know you wont see. I know you dont care to see. I hope you can see.
—–Quentin 那歇丝底里却又无助的独白呓语一般不断的重复着.他似乎重又点燃了我的热情.终究是无用的.明天还是一样.没有任何变化.你看不到的.你不在乎.因为
                                      ———-只有重的人才会热切的羡慕和欣赏轻的人写的文字.我的世界是沉重的,强大的引力压的透不过气来.我是一只                                                 大恐龙.你呢.你呢.你呢.你不在.
Aug 25 Mon sunny

9:00-10:20: Plant growth assay Day-3 count.
10:20-11:40: PCR set up.
11:40-12:30: Wash plates.
13:30-13:55: Prepare gel.
14:00-15:25: Run gel.
15:25-16:10: Collect leaf tissues and freeze in liquid nitrogen.
16:10-16:30: Gel result checking (unique band in 5F8 E/P).
16:30-17:05: 5F8 DNA eXtraction.
17:05-17:35: PCR set up with the DNA eXtracted.
17:25-18:30: Finish collecting and freezing.

Day 66

———-陀思妥耶夫斯基陀思妥耶夫斯基的的小说小说跟他跟他名字名字一样一样冗长冗长而而难以下咽难以下咽.不过不过<群魔群魔>还是还是能看的能看的.
———-aЯa/aЯa/aЯa/aЯa/aЯa
———-113×3… Possibly.

Aug 22 Fri sunny

9:00-10:00: Run gel using PCR products from yesterday.
10:00-11:35: Prepare Col-0 seedling plates.
10:40-10:55: Gel result checking.
11:35-12:00: Wash plates. ESTM retest (8/15) check.
12:00-14:15: Lab member gathering: Sushi on Bloor.
14:15-14:45: Make dilution series for bac. strains to reconfirm bac. growth.
14:45-16:30: Plant growth assay Day-3 set up.

Day 65

Aug 21 Thur sunny

———-毫无毫无内容内容的的空白空白的的思念思念无疑无疑是是最伤最伤身体身体的的了了.其中其中的的人人或或事事不过不过是个是个抽象抽象的的符号符号存在存在脑海脑海里里.时空时空没法没法连续连续,只有只有一个个一个个现时现时的的片段片段.这这是一种是一种强迫强迫,也是也是一种一种苛责苛责.就像是就像是空着空着胃胃呕吐呕吐一般一般,明知明知无能为力无能为力,却也却也只是要求只是要求意识意识的的安慰安慰罢了罢了.经久经久难免难免愈加愈加伤痛伤痛.

———-天空天空微亮微亮的的时候时候,究竟究竟还是还是睡过去睡过去了了.

———-当一个人当一个人决心决心把把有限有限的的生命生命投入到投入到无尽无尽的的事业事业中去中去的的时候时候,他他便成了便成了西西弗斯西西弗斯的的同伴同伴.但是但是,谁谁又能够又能够说他说他是是不不幸福幸福的的呢呢?他的他的幸福幸福是他是他自己自己的的事事,他的他的痛苦痛苦也是他也是他自己自己的的事事.他他便成了便成了世界世界.时间时间也因此也因此凝固了凝固了.爱爱虽然虽然是是永远永远达不到达不到的的彼岸彼岸,但但可能性可能性是是无限的无限的.爱爱达到了达到了永恒永恒.

9:00-10:25: Prepare MS &KB media and go autoclave.
10:30-10:50: ESTM plant growth assay Day-0 check.
10:50-12:00: PCR set up.
13:00-14:20: ESTM retest.
14:20-17:00: ESTM growth assay Day-3 set up.
17:00-18:00: ESTM 35~38 result checking and make dilution series for positives and plate out.

Day 64

———-麻痹神经麻痹神经逃避思想逃避思想的的最好最好办法办法莫过于莫过于简单简单重复重复的的劳动劳动,比如比如rack tips,比如比如wash plates.

Aug 20 Wed overcast

9:00-12:55: ESTM 32&39 inoculation expt.
14:00-17:00: ESTM 40&41 inoculation expt.
17:00-17:20: Restreak bac. strains for retesting.
17:20-17:40: Rack tips.

Day 63

———–给给植物植物注射注射的的时候时候,我我仿佛仿佛以以一种一种奇妙奇妙的的角度角度注视注视着着死亡死亡.一切一切都是都是那么那么透明透明,一切一切都都变得变得没有意义没有意义.仿佛仿佛在在月球月球表面表面,轻轻的的让人让人透不过气透不过气.

———–存在主义者存在主义者的的爱情爱情是是不负责任不负责任的的爱情爱情.因为因为他们他们太太负责任负责任,对对自己自己,也对也对所有人所有人.

———–ARE U BORED WITH ME

Aug 19 Tue overcast
9:00-9:30: Prepare gel.
9:30-10:15: Restreak ESTM 32,39~41.
10:15-10:40: Run gel using PCR products obtained from yesterday.
10:40-11:15: Wash plates.
11:15-11:40: Gel result check.
12:30-14:30: ESTM growth assay set up: Inoculate Col-0 w/ .0002 20G3/26B5/524.
14:30-16:00: Transplant Col-0 (three trays).
16:00-16:45: ESTM growth assay Day-0 set up as described before.
16:45-17:05: Restreak bac. strains for retesting from glycerol stocks.
17:10-18:20: PCR set up for both the plasmid DNA redo and ESTM DNA redo (with lower annealing temp).
18:20-18:40: Rack tips.