Day 7

May 27 Tue overcast
9:00-11:00: Make freeze stock for transposon inoculated bacteria.
11:00-12:00: Make ecotype stock (seeds from harvesting; two 96-well plates).
12:35-13:00: Sterilize plates w/ ecotype and seeds from ML1 insertion line by chlorin gas for three and half hours.
13:30-14:50: Wash plant trays.
15:00-16:00: Harvest seeds (one tray).
16:00-16:30: Rinse 96-well plates.
16:30-19:25: Make sterile plant stock from ecotype and ML1 insertion line 1-60.

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