Jun 11 Wed overcast 9:00-17:00: Make ESTM 5&6 inoculation plates as described before.17:05-17:50: Make 10, 100, 1,000 and 10,000 dilution series for following inoculated solutions from ESTM 3 plates and plate and aliquot of each dilution onto a petri dish to observe bacteria growth: […]

Jun 10 Tue overcast 9:00-10:00: Set up ML1 activation line 122~181.10:00-12:00: WHMIS safety training session.13:00-14:35: Make Col-0 seedling stocks in 96-well plates(18).14:35-15:35: Make two ecotype seedling stocks.15:40-18:25: Make ML1 activation line 122~181 media stocks.

Jun 9 Mon Sunny w/ afternoon t-storm 9:00-9:50: Wash 96-well plates.9:50-10:30: Plate the bacteria from glycerol stocks ESTM 5&6 to new plates.10:30-11:45: Transplant Col-0 from pot to tra with soil (2).11:45-12:10: Wash and rinse 96-well plates.13:00-13:40: Bacteria colony count for leaf tissue plate prepared […]

9:00-10:15: Treat seedling plates with constitutive promoter transposon inserted with DMSO or Smex (two plates for each kind).                     Smex is light sensitive.10:15-10:50: ESTM 1&2 inoculation line result recording.10:50-11:55: Make 10, 100 and 1,000 dilution of selected ESTM line wells and to observe bacteria growth by […]

Jun 5 Thur overcast w/ afternonn shower   9:00-11:20: Make Col-0 stocks in 96-well plates (21 prepared). 11:30-13:40: Make sterile stocks for Col-0, NahG and six other mutants in 96-well plates. 14:10-14:40: Transplant excess Col-0 seeds and ecotype #8,10 and 74 to soil pots and […]