Jun 18 Wed overcast   9:00-10:30: ESTM 5&6 inoculation plate recording for plants w/ non-bleached phenotype.                   Make dilution series for these wells as well as the negative controls to observe bacteria growth. 10:30-12:00: ESTM 7&8 inoculation prep: make .004 bacteria plates. 12:40-15:30: ESTM […]

Jun 16 Tue overcast w/ shower sometime in the day 9:00-9:50: ML1 activation line set up.9:50-11:00: Make ecotype 7&8 stocks.11:00-11:55: Make ecotype retest seed stock for sterilization.12:45-14:05: Prepare KB, LB and MS agar media and autoclave.14:05-14:25: Make ESTM 7&8 replica plates.14:25-15:05: Wash 96w plates.15:05-16:30: […]

Jun 16 Mon overcast w/ afternoon shower   9:00-10:30: Wash and rinse 96w plates. 10:30-11:50: Ecotype 1&2 retest set up (two replicates): Col-0, #5, #10 and La-er. 12:50-15:50: Transplant ecotype #74, #10, #8 and Col-0 from pot to tray of soil.                     Wash and […]

Jun 13 Fri sunny w/ evening t-storm           ——————- a day being doomed 9:00-10:30: Treat ML1 61~121 line with two micro litres of DMSO or Smex (two plates each) for each well. Then stick around and store in dark for six hours or more.10:30-11:40: Set up […]

Jun 12 Thur overcast 9:00-10:20: Wash plates.10:30-12:00: Wash and dry trays, sleeves and pots.12:45-13:45: Observe inoculated Eco1&2 plates (prepared last Fri)under dissecting microscope;                       Observe inoculated ML1 1~60 +Smex line under microscope to look for bleached phenotype.13:45-14:40: Make MS+MES media stocks for Col-0 (6) and ecotype […]