Aug 12 Tue sunny 9:00-10:15: Restreak ESTM 35~38 from glycerol stocks.10:15-11:00: PCR set up.11:00-13:10: Collect leaf tissue from ecotype mutants and freeze in liquid nitrogen.13:10-13:25: Prepare gel.14:00-14:30: Run gel for forty mins.14:30-15:20: Finish up collecting leaf tissue.15:20-16:00: Gel result checking.16:00-16:50: Gradient PCR set up.

Aug 11 Mon sunny 9:00-10:30: Prepare KB&LB media and go autoclaving.10:30-11:05: PCR set up.Sterilize Col-0 seedling plates.11:05-11:35: Wash plates.11:35-12:00: Autoclave out.12:00-12:35: Restreak bacteria strains for retest.13:25:-13:55: PCR done. Run gel for 40 mins.13:55-14:55: Prepare MS media stocks for Col-0 seedling plates.14:55-15:30: Electrophoresis results checking.

Aug 8 Fri sunny 9:00-11:30: Prepare Col-0 seedling plates.11:30-12:00: Wash plates.12:00-12:25: Prepare gel for electrophoresis.13:00-13:35: ESTM retest recording.13:35-14:35: Wash plates./Electrophoresis result checking.14:40-15:10: PCR using universal vector.15:10-18:00: Wash trays.18:00-18:25: ESTM retest (Day 3): Make dilution series and plate out.

Aug 7 Thur sunny 9:00-10:00: Rack tips.10:00-13:10: ESTM 31&32 inoculation expt.14:00-16:30: ESTM 33&34 inoculation expt.16:30-18:30: ESTM retest: inoculate Col-0 plates with .004 bacteria strains.18:30-19:05: ESTM retest (Day 1): Make dilution series and plate out.

Aug 6 Wed sunny 9:00-10:00: Restreak ESTM 31~34.10:00-11:10: PCR set up for 8B7 (with universal vector).11:10-11:30: Wash plates.11:30-12:00: Paper review.13:00-13:30: Paper review. Wash plates.13:30-14:00: Run electrephoresis for PCR products.14:00-14:30: ESTM 27~30 checking.14:30-15:35: ESTM retest: make dilution series and plate out.15:35-16:15: Second PCR set up […]