Jun 25 Wed sunny 9:00-13:40: Inoculate Col-0 plates w/ ESTM 9&10 at concentration of .004 A (three replica each).14:00-17:00: Set up ESTM line growth assay w/ media from seedling at concentration of .15 A and store in the 28 C shaker.17:00-17:40: Inoculate ecotype plates […]

9:00-10:00: Replicate ESTM 9&10.10:00-12:40: Inoculate Col-0 plates with eight strains from ESTM line prepared at .004 concentration.13:00-13:55: Wash 96w plates.

9:00-15:00: ESTM growth assay set up: make 0.15 plate for each one tested in KB media.15:00-15:30: Restreak ESTM 9&10.15:30-17:10: Make .004 plate of pathogen #524 in MS media to inoculate ECO #6 and #500 for DMSO and Smex treated plates.17:10-17:30: ESTM growth assay record […]

Jun 20 Fri overcast 9:00-11:00: Make Col-0 stock in 96w plates (20).11:00-12:00: Paper review.12:40-14:40: Make bacteria growth media from Col-0 media stock.14:40-15:10: Wash 96w plates.15:10-16:10: Make .004 pathogen #524 media and inoculate it to Ecotype #5.16:10-17:00: Restreak bacteria from ESTM plates with non-bleached phenotype to petri dish.

Jun 19 Thur overcast 9:00-10:00: eXamine ML161~121 w/ DMSO or Smex plates and ecotype 3&4 for non-bleached phenotype.10:00-11:00: Lab meeting resumed.11:00-11:40: Meeting with Darrell.11:40-12:25: Rack tips and record examining results in computer.13:00-13:50: Rack tips.13:50-14:35: Wash 96w plates.14:35-15:20: Rack tips.