Aug 5 Tue sunny w/ eveing storm 9:00-10:00: Wash plates.10:00-10:30: Restreak ESTM 31~34.10:30-10:40: Col-0 seedling plates sterilization.10:40-11:05: ESTM growth assay recording.11:20-12:00: Restreak bacteria strains for retesting.13:00-14:50: Transplant Col-0 and Ecotype #8/10/74.14:50-15:30: Prepare MS media stocks for Col-0 seedling plates.15:30-16:10: shaker checking.
Category: 日记独白
Day 53
Aug 1 Fri sunny 9:00-11:00: Prepare Col-0 seedling plates.11:00-11:20: Count colonies from ESTM growth assay Day 0.11:30-12:20: Wash trays.12:05-12:50: Prepare KB media and go autoclaving.13:30-14:00: Autoclave out.14:00-15:00: Wash plates.15:00-15:50: Prepare KB media plates with antibiotics.16:00-16:15: ESTM growth assay Day 3: Make dilution series and […]
Day 52
July 31 Thur sunny 9:00-10:50: ESTM retesting: Make dilution series.11:00-13:25: Inoculate plates with .004 baceteria strains.13:30-14:45: Wash plates.14:45-15:30: Rack tips.16:00-16:25: ESTM retest: Make dilution series for 8B7/10B7/524.
Day 51
July 30 Wed sunny 9:00-13:10: ESTM 27&28 inoculation expt.13:40-16:35: ESTM 29&30 inoculation expt.16:40-18:00: Make dilution series for bacteria solution interested and plate out on petri dish.18:00-18:15: Restreak bacteria strains for retesting.
Day 50
July 29 Tue sunny 9:00-9:50: Wash plates.9:50-10:30: Restreak bacteria strains for retesting.10:30-11:00: ESTM Growth Assay in Seedling Media recording (22 hpi).11:00-11:30: Rack tips.11:30-12:00: Restreak 8B7/10B7/524 for retesting.13:00-13:25: Restreak ESTM 27~30.13:30-15:55: Harvest seeds.16:00-17:00: Prepare MS media for Col-0 seedling plates.